Table 3:
Primary antibodies used to characterise EiTiX embryos
| Marker | Spatial expression | Antibody | Dilution used |
|---|---|---|---|
| Ap2γ | ExE | R&D Systems, cat. no. AF5059 (RRID: AB_2255891, https://scicrunch.org/resolver/RRID:AB_2255891) |
1:500 |
| Brachyury | Primitive streak | R&D Systems, cat. no. AF2085 (RRID: AB_2200235, https://scicrunch.org/resolver/RRID:AB_2200235) |
1:500 |
| Cdx2 | ExE | BioGenex, cat. no. MU392-UC (RRID: AB_2335627, https://scicrunch.org/resolver/RRID:AB_2335627) |
1:500 |
| Abcam, ab76541 (RRID: AB_1523334, https://scicrunch.org/resolver/RRID:AB_1523334) |
1:500 | ||
| Cerberus1 | AVE | R&D Systems, cat. no. MAB1986 (RRID: AB_2275974, https://scicrunch.org/resolver/RRID:AB_2275974) |
1:500 |
| Dkk1 | AVE | R&D Systems, cat. no. AF1096 (RRID: AB_354597, https://scicrunch.org/resolver/RRID:AB_354597) |
1:1000 |
| Eomes | ExE | Abcam, cat. no. ab23345 (RRID: AB_778267, https://scicrunch.org/resolver/RRID:AB_778267) |
1:500 |
| FoxA2 | Definitive endoderm | Cell Signaling Technology, cat. no. 8186 (RRID: AB_10891055, https://scicrunch.org/resolver/RRID:AB_10891055) |
1:200 |
| Gata4 | VE, heart | Santa Cruz Biotechnology, cat. no. sc-9053 (RRID: AB_2247396, https://scicrunch.org/resolver/RRID:AB_2247396) |
1:500 |
| Gata6 | VE | R&D Systems, cat. no. AF1700 (RRID: AB_2108901, https://scicrunch.org/resolver/RRID:AB_2108901) |
1:500 |
| Hand1 | Chorion | Santa Cruz Biotechnology, cat. no. sc-390376 (RRID: AB_2935660, https://scicrunch.org/resolver/RRID:AB_2935660) |
1:100 |
| Lefty | AVE | R&D Systems, cat. no. AF746 (RRID: AB_355566, https://scicrunch.org/resolver/RRID:AB_355566) |
1:1000 |
| Myh2 | Heart | R&D Systems, cat. no. MAB4470 (RRID: AB_1293549, https://scicrunch.org/resolver/RRID:AB_1293549) |
1:500 |
| Nkx2.5 | Heart | R&D Systems, cat. no. AF2444 (RRID: AB_355269, https://scicrunch.org/resolver/RRID:AB_355269) |
1:500 |
| Oct3/4 | EPI | Santa Cruz Biotechnology, cat. no. sc-5279 (RRID: AB_628051, https://scicrunch.org/resolver/RRID:AB_628051) |
1:250 |
| Otx2 | Fore-/mid-brain | R&D Systems, cat. no. AF1979 (RRID: AB_2157172, https://scicrunch.org/resolver/RRID:AB_2157172) |
1:500 |
| Runx1 | Haematopoietic progenitor | Abcam, cat. no. ab92336 (RRID: AB_2049267, https://scicrunch.org/resolver/RRID:AB_2049267) |
1:500 |
| Sox1 | Neuroepithelium | Cell Signaling Technology, cat. no. 4194 (RRID: AB_1904140, https://scicrunch.org/resolver/RRID:AB_1904140) |
1:500 |
| Sox2 | EPI, neuroepithelium | Santa Cruz Biotechnology, cat. no. sc-365823 (RRID: AB_10842165, https://scicrunch.org/resolver/RRID:AB_10842165) |
1:200 |
| Thermo Fisher Scientific, cat. no. 14–9811-82 (RRID: AB_11219471, https://scicrunch.org/resolver/RRID:AB_11219471) |
1:500 | ||
| Sox17 | Definitive endoderm | R&D Systems, cat. no. AF1924 (RRID: AB_355060 https://scicrunch.org/resolver/RRID:AB_355060) |
1:500 |
ExE: extraembryonic ectoderm, AVE: anterior visceral endoderm, VE: visceral endoderm, EPI: epiblast