| Composition | Volume (20ml) | Final concentration |
|---|---|---|
| N2B27 | 20ml | |
| CHIR99021 (10mM stock) | 6µl | 3µM |
| PD0325901 (10mM stock) | 2µl | 1µM |
| Mouse LIF (10µg/ml stock) | 20µl | 10ng/ml |
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