| Composition | Volume (600ml) | Final concentration |
|---|---|---|
| DMEM | 500ml | |
| Inactivated FBS | 90ml | 15% (vol/vol) |
| GlutaMAX | 6ml | 2mM |
| Pen/strep | 6ml | 1% (vol/vol) |
| NEAA | 6ml | 1% (vol/vol) |
| Sodium pyruvate | 6ml | 1mM |
| Beta-mercaptoethanol | 1.2ml | 0.1mM |
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