| Composition | Volume (50ml) | Final concentration |
|---|---|---|
| Advanced DMEM/F12 | 50ml | |
| GlutaMAX | 500µl | 2mM |
| Pen/strep | 125µl | 0.25% (vol/vol) |
| ITS-X | 500µl | 1% (vol/vol) |
| Composition | Volume (50ml) | Final concentration |
|---|---|---|
| Advanced DMEM/F12 | 50ml | |
| GlutaMAX | 500µl | 2mM |
| Pen/strep | 125µl | 0.25% (vol/vol) |
| ITS-X | 500µl | 1% (vol/vol) |