Range characterisation of the three dsRNA quantification methods evaluated (HPLC, dot blot and ELISA) in terms of limit of detection (LOD), limit of quantification (LOQ), decision limit (CCα) and the detection capability (CCβ).
| Parameter | Analytical method | |||||
|---|---|---|---|---|---|---|
| HPLC | Dot blot | ELISA | ||||
| x̄ (g L−1) | σ (g L−1) | x̄ (g L−1) | σ (g L−1) | x̄ (g L−1) | σ (g L−1) | |
| LOD | 1.41 × 10−2 | 1.78 × 10−3 | 2.01 × 10−1 | 5.96 × 10−2 | 1.51 × 10−5 | 3.21 × 10−6 |
| LOQ | 4.27 × 10−2 | 5.40 × 10−3 | 6.09 × 10−1 | 1.81 × 10−1 | 4.56 × 10−5 | 9.73 × 10−6 |
| CCα | 9.95 × 10−3 | 1.26 × 10−3 | 1.42 × 10−1 | 4.21 × 10−2 | 1.06 × 10−5 | 2.27 × 10−6 |
| CCβ | 1.70 × 10−2 | 2.14 × 10−3 | 2.42 × 10−1 | 7.17 × 10−2 | 1.81 × 10−5 | 3.86 × 10−6 |