Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | Kidney; Embryo | ATCC | CRL-3216 | HEK293T |
| Cell line (Homo sapiens) | Uterus; Cervix, Adenocarcinoma |
ATCC | CCL-2 | HeLa |
| Cell line (Homo sapiens) | Large intestine; Colon Adenocarcinoma |
ATCC | CCL-228 | SW480 |
| Transfected construct human | MA-T-gRNA-RIP1 | Addgene | 48138 | RIPK1-KO |
| Transfected construct human | Cas9-GFP pSp Cas9(BB)–2A-GFP |
RIPK1-KO | ||
| Transfected construct human | pcDNA3.1-F[1] -LKB1-F[2] |
LKB1-KinCon | ||
| Transfected construct human | pcDNA3.1- F[1]-LKB1-K78I-F[2] |
LKB1-KinCon (K78I) | ||
| Transfected construct human | pcDNA3.1-F[1] -LKB1-D176N-F[2] |
LKB1-KinCon (D176N) | ||
| Transfected construct human | pcDNA3.1-F[1] -LKB1-D194N-F[2] |
LKB1-KinCon (D194N) | ||
| Transfected construct human | pcDNA3.1-F[1]-LKB1-R74A-F[2] | LKB1-KinCon (R74A) | ||
| Transfected construct human | pcDNA3.1-F[1]-LKB1-W308C-F[2] | LKB1-KinCon (W308C) | ||
| Transfected construct human | pcDNA3.1-F[1] -LKB1-K48R-F[2] |
LKB1-KinCon (K48R) | ||
| Transfected construct human | pcDNA3.1-F[1] -STRADα-F[2] |
STRADα-KinCon |
||
| Transfected construct human | pcDNA3.1-LKB1-3xFlag | LKB1-3xFlag |
||
| Transfected construct human | pcDNA3.1- STRADα–3xFlag |
STRADα–3x Flag |
||
| Transfected construct human | pcDNA3.1- STRADα- H231A/F233A-3xFlag |
STRADα–3xFlag (H231A /F233A) | ||
| Transfected construct human | pcDNA3.1- F[1]-MLKL-F[2] |
MLKL- KinCon |
||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1- S14/S15/S166A-F[2] |
RIPK1-KinCon (S14A/S15A/S166A) | ||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1- S14/S15/S166E-F[2] |
RIPK1-KinCon (S14E/S15E/S166E) | ||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1-K45A-F[2] |
RIPK1-KinCon (K45A) |
||
| Transfected construct human | pcDNA3.1-F[1]-RIPK1-D324H-F[2] | RIPK1-KinCon (D324H) | ||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1-D324A-F[2] |
RIPK1-KinCon (D324A) |
||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1-D324E-F[2] |
RIPK1-KinCon (D324E) | ||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1-C601Y-F[2] |
RIPK1-KinCon (C601Y) |
||
| Transfected construct mouse | pcDNA3.1- F[1]-CDK6-F[2] |
CDK6-KinCon | ||
| Transfected construct mouse | pcDNA3.1- F[1]-CDK6-R31C-F[2] |
CDK6-KinCon (R31C) |
||
| Transfected construct mouse | pcDNA3.1- F[1]-CDK4-F[2] |
CDK4-KinCon | ||
| Transfected construct mouse | pcDNA3.1- CDK6-F[1] |
CDK6-PPI reporter | ||
| Transfected construct mouse | pcDNA3.1- CDK6-R31C-F[1] |
CDK6-PPI reporter (R31C) |
||
| Transfected construct mouse | pcDNA3.1- CDK4-F[1] |
CDK4-PPI reporter | ||
| Transfected construct mouse | pcDNA3.1- CDK4-R24C-F[1] |
CDK4-PPI reporter (R24C) |
||
| Transfected construct mouse | pcDNA3.1- p16INK4a-F[2] |
p16INK4a-PPI reporter | ||
| Transfected construct mouse | pcDNA3.1- p16INK4a-P40L-F[2] | p16INK4a-PPI reporter (P40L) |
||
| Transfected construct human | pcDNA3.1- F[1]-MEK1-F[2] |
MEK1-KinCon | ||
| Transfected construct human | pcDNA3.1- F[1]-MEK1-K57E-F[2] | MEK-KinCon (K57E) |
||
| Transfected construct human | pcDNA3.1- F[1]-PKAc-F[2] |
PKAc-KinCon | ||
| Transfected construct human | pcDNA3.1- F[1]-PKAc-L206R-F[2] |
PKAc-KinCon (L206R) |
||
| Transfected construct human | pcDNA3.1- MO25-3xFlag |
MO25-3xFlag | ||
| Transfected construct human | pcDNA3.1- F[1]-BRAF-F[2] |
BRAF-KinCon | ||
| Transfected construct human | pcDNA3.1- F[1]-BRAF-V600E-F[2] |
BRAF-KinCon (V600E) |
||
| Transfected construct human | pcDNA3.1- F[1]-RIPK1-F[2] |
RIPK1-KinCon | ||
| Transfected construct human | pcDNA3.1- F[1]-RIPK2-F[2] |
RIPK2-KinCon | ||
| Transfected construct human | pcDNA3.1- F[1]-RIPK3-F[2] |
RIPK3-KinCon | ||
| Antibody | GAPDH Rabbit monoclonal |
Cell Signaling | 2118 | 1:10000 |
| Antibody | AMPKα Rabbit monoclonal |
Cell Signaling | 2532 | 1:1000 |
| Antibody | Phospho- AMPKα(Thr172) Rabbit monoclonal |
Cell Signaling | 2535 | 1:1000 |
| Antibody | LKB1 Rabbit monoclonal |
Cell Signaling | 3047 | 1:1000 |
| Antibody | Vinculin Rabbit monoclonal |
Cell Signaling | 4650 | 1:1000 |
| Antibody | RIP XP Rabbit monoclonal |
Cell Signaling | 3493 | 1:1000 |
| Antibody | RIP3 Rabbit monoclonal |
Cell Signaling | 13526 | 1:1000 |
| Antibody | CDK6 Mouse monoclonal |
Cell Signaling | 3136 | 1:1000 |
| Antibody | FLAG-M2 Mouse monoclonal |
Sigma-Aldrich | F3165 | 1:1000 |
| Antibody | Renilla Luciferase Rabbit monoclonal |
Abcam | Ab185926 [EPR17792] | 1:10000 |
| Antibody | Renilla Luciferase clone 1D5.2 Mouse monoclonal |
Millipore | MAB4410 | 1:1000 |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-50767 | Palbociclib PD 0332991 |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-16297A | Abemaciclib LY2835219 |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-15777 | Ribociclib LEE011 |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-18972 | PLX8394 |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-114492 | GSK-547 |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-14622A | Necrostatin 1 S |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-13064 | Cobimetinib |
| Drug, chemical compound | Inhibitor | MCE Med Chem Express | HY-P7090A | TNFα |
| Drug, chemical compound | Inhibitor | MCE | HY-16658 | z-VAD(OMe)-FMK |