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. 2024 Jun 20;2024(3):hoae042. doi: 10.1093/hropen/hoae042

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© The Author(s) 2024. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Knockdown of monoamine oxidase downregulates genes related to lipid metabolism. (A) Principal component analysis of the RNA-seq data from hESCs after transfection with siNC, siMAOA, or siMAOB followed by stimulation with 8-Br-cAMP and MPA for 3 days (corresponding to NC, siMAOA, and siMAOB groups respectively). NS, transfected siNC without 8Br-cAMP+MPA stimulation (n=3). (B) Number of DEGs between the different comparisons. (C and D) GO enrichment analysis of downregulated genes in decidualized hESCs after knockdown of MAOA or MAOB. (E) Clustered heatmap of average expression of representative downregulated genes related to lipid metabolism in decidualized hESCs after knockdown of MAOA or MAOB. (F) Verification of the expression of genes related to lipid metabolism by real-time PCR in decidualized hESCs after knockdown of MAOA or MAOB, normalized to the 18s rRNA (n=3, *P<0.05, **P<0.01, ***P<0.001, statistically significant by ordinary one-way ANOVA). PCA, principal component analysis; hESCs, human endometrial stromal cells; MAOA, monoamine oxidase A; MAOB, monoamine oxidase B; 8Br-cAMP, 8-bromoadenosine 3′,5′-cyclic monophosphate; MPA, medroxyprogesterone acetate; DEGs, differentially expressed genes; GO, gene ontology.