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. 2024 Jun 20;2024(3):hoae042. doi: 10.1093/hropen/hoae042

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© The Author(s) 2024. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Phosphatidylcholine rescues serotonin-suppressed human endometrial stromal cell decidualization. (A) ELISA was used to detect the expression of serotonin in the cell supernatant of hESCs after knockdown of MAOA or MAOB followed by stimulation with 8-Br-cAMP and MPA for 3 days (n=4, **P<0.01). (B–D) Real-time PCR analysis of the PRL, IGFBP1, and FOXO1 transcriptional expression levels in hESCs treated with different doses of serotonin followed by stimulation with 8-Br-cAMP and MPA for 3 days (n=3, *P<0.05, **P<0.01, statistically significant by Student’s t-test). (E) The protein expression levels of PEMT in hESCs after transfection with siNC, siMAOA, or siMAOB followed by stimulation with 8-Br-cAMP and MPA for 3 days. (F) Real-time PCR analysis of the PEMT transcriptional expression levels in hESCs treated with serotonin followed by stimulation with 8-Br-cAMP and MPA for 3 days (n=3, *P<0.05, **P<0.01, statistically significant by Student’s t-test). (G) The protein expression levels of PEMT and FOXO1 in hESCs treated with serotonin followed by stimulation with 8-Br-cAMP and MPA for 3 days. (H) Synthesis of phosphatidylcholine from phosphatidylethanolamine catalyzed by PEMT. (I) The protein expression levels of FOXO1 in hESCs treated with 30 μM serotonin and different doses of PC followed by stimulation with 8-Br-cAMP and MPA for 3 days. (J–L) Real-time PCR analysis of the PRL, IGFBP1, and FOXO1 transcriptional expression levels in hESCs treated with 30 μM serotonin and different doses of PC followed by stimulation with 8-Br-cAMP and MPA for 3 days (n=4, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, statistically significant by Student’s t-test). hESCs, human endometrial stromal cells; MAOA, monoamine oxidase A; MAOB, monoamine oxidase B; PRL, prolactin; IGFBP1, insulin-like growth factor binding protein 1; FOXO1, forkhead box O1; 8Br-cAMP, 8-Bromoadenosine 3′,5′-cyclic monophosphate; MPA, medroxyprogesterone acetate; PEMT, phosphatidylethanolamine N-methyltransferase; PC, phosphatidylcholine; PE, phosphatidylethanolamine.