FIG. 1.

Protein composition of the assembly intermediates for the 2195H mutants. Lysates (lanes L) from vaccinia virus-infected cDNA-transfected cells and sucrose gradient fractions corresponding to the different assembly intermediates (5S protomers, 14S pentamers, 80S empty capsid, 110S encapsidation intermediate, and 150S virion) were analyzed by SDS–10% PAGE for the wild-type poliovirus and 2195H mutants. T7, vaccinia virus-T7-infected cell lysates; PVM (WT), vaccinia virus-infected cells transfected with the wild-type poliovirus cDNA plasmid. The migration of the poliovirus proteins and the prestained molecular mass markers (in kilodaltons; Bio-Rad) are indicated.