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. 2024 Aug 1;14(8):e1785. doi: 10.1002/ctm2.1785

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© 2024 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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CPT1A deficiency in macrophage contributes to more severe lung injury in the ALI mouse model. (a) Cre⁻ CPT1A ^fl/fl and Cre⁺ CPT1A ^fl/fl mice were administrated nasally with PBS or LPS (10 mg/kg). After 24 h, mice were euthanised and Balf was collected and estimated for total cell number, macrophage, neutrophil and lymphocyte numbers. n = 6 biologically independent samples. Balf total cell, **p = .0044, ****p < .0001. Macrophage, ****p < .0001. Neutrophil, ***p = .0002, ****p < .0001. Lymphocyte, **p = .002, ****p < .0001. (b, c) Secretion of cytokines IFN‐γ, IL‐6, G‐CSF, CXCL1/KC and IL‐10 in Balf and serum was assessed by ELISA kits. n = 6 biologically independent samples. (b) *p = .0212, **p = .0017, ****p < .0001. (c) *p = .0368 (Cre⁻ CPT1A ^fl/fl+Con group vs. Cre⁻ CPT1A ^fl/fl+LPS group), *p = .0116 (Cre⁻ CPT1A ^fl/fl+LPS group vs. Cre⁺ CPT1A ^fl/fl+LPS group), **p = .0046, *p = .0448, ****p < .0001. (d, e) Quantitative analysis of immunoblotting assay identifying the activation of NLRP3/Caspase‐1 pathway for lung inflammation evaluation. n = 6 biologically independent samples. (d) ***p = .0002 (Cre⁻ CPT1A ^fl/fl+Con group vs. Cre⁻ CPT1A ^fl/fl+LPS group), ***p = .0009 (Cre⁻ CPT1A ^fl/fl+LPS group vs. Cre⁺ CPT1A ^fl/fl+LPS group), ****p < .0001. (e) **p = .0086, ****p < .0001. (f) Representative pictures of H&E‐stained lung tissue sections from each group. Scale bars in the upper panel, 200 µm; scale bars in the lower panel, 50 µm. Lung injury score was determined by 5 pathophysiological features. n = 6 biologically independent samples. ****p < .0001. (g) Immunohistochemical staining and quantitative analysis of IL‐10 in Cre⁻ CPT1A ^fl/fl and Cre⁺ CPT1A ^fl/fl mice. Red arrowheads indicate IL‐10‐expressed macrophage within lungs. n = 30 each group from 6 biologically independent samples. ****p < .0001. Scale bars in the upper panel, 200 µm; scale bars in the lower panel, 50 µm. Data are presented as mean ± SEM and analysed with a 95% confidence interval. p Values were calculated using one‐way ANOVA followed by Bonferroni's post hoc test.