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. 2024 May 16;15(4):1388–1403. doi: 10.1002/jcsm.13484

Figure 7.

Figure 7

FGF7 delayed myogenic cell senescence. (A) Immunofluorescence staining with γH2AX (green) and DAPI (blue) was detected in synchronously cultured passage 15 LD‐MuSCs and SOL‐MuSCs, and the nuclear area (n = 200 nuclei) and ratio of γH2AX+ cells (n = 5 samples) were quantified. The scale bar is 10 μm. (B) Immunofluorescence staining with γH2AX (green) and DAPI (blue) was detected in porcine MuSCs treated with 10‐ng/mL FGF7 recombinant protein, 20‐g/L d‐gal and 20‐g/L d‐gal + 10‐ng/mL FGF7, and the nuclear area (n = 200 nuclei) and ratio of γH2AX+ cells (n = 4 samples) were quantified. The scale bar is 10 μm. (C) Immunofluorescence staining with EdU (red) and Hoechst (blue) was detected in C2C12 treated with 10‐ng/mL FGF7 recombinant protein, 20‐g/L d‐gal and 20‐g/L d‐gal + 10‐ng/mL FGF7, and the ratio of EdU+ cells was quantified. The scale bar is 100 μm (n = 5) (*P < 0.05, ** P < 0.01 and *** P ≤ 0.001). Data are presented as mean ± SD.