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. 2024 Jul 19;14:1440650. doi: 10.3389/fonc.2024.1440650

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Copyright © 2024 Wang, Ostergaard, Kang, Sagong, Twite, Vargas-Morales and Gordon

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hypoxanthine in HPLM media contributes to 6-TG resistance. (A–E) 6-TG dose-response curves for leukemia cells (NALM-6, A; REH, B; SEM, C; Jurkat, D; CEM, E) pre-incubated in HPLM or RPMI for 48 hours prior to the addition of 6-TG. After an additional 48 hours of drug treatment, leukemia cell viability was assessed using the CellTiter-Glo Luminescent Cell Viability Assay. Error bars represent the mean ± SD of three technical replicates. (F–J) 6-TG dose-response curves for leukemia cells (NALM-6, F; REH, G; SEM, H; Jurkat, I; CEM, J) pre-incubated in HPLM for 48 hours prior to the addition of 6-TG +/- hypoxanthine 10 μM. After an additional 48 hours of drug treatment, leukemia cell viability was assessed using the CellTiter-Glo Luminescent Cell Viability Assay. Error bars represent the mean ± SD of three technical replicates.