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. 2024 Aug 1;10:106. doi: 10.1038/s41378-024-00733-1

Fig. 2. Calcium monitoring micro-total analysis system (CAM-μTAS) to quantify calcium dynamics in microglial cells.

Fig. 2

a A fabricated CAM-μTAS, including the lifting gate valve and a scan electron microscopy image of the Quake valve. b CAD design of the CAM-μTAS, including lifting gate valves, microfluidic rectifiers, and a quake valve. c A feature illustration of the CAM-μTAS. The device combines a series of lifting gate valves into a micropump to automatically and precisely meter cell culture media changes, load calbryte 520-Am, and eliminate backflow. The CAM-μTAS leverages the flow-blocking capability of the Quake valve to generate a concentration gradient, which enables a biomimetic delivery of inflammatory signals to the cells