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A concentration gradient was generated to deliver pro-inflammatory cytokines (IL-1β [10 ng/mL], IL-6 [10 ng/mL], TNF-α [5 ng/mL], and ATP [200 μM]) to the cells. As seen in the above fluorescence plot, the decreasing concentration of the signal from the signal chamber to the cell culture chamber makes the CAM-μTAS biomimetic. Dextran was added to the cytokine solution for fluorescent imaging purposes
