FIG. 5.

BALB/c mice were injected intramuscularly with 100 μg of plasmid DNA three times at 0, 2, and 4 weeks. Anti-Gag antibodies were measured at week 6. Sera were collected from each mice group injected with the different DNA constructs, pooled, and analyzed by immunoblot, using purified viral particle Gag p55 (A) or purified mature HIV-1 virions (B). Vaccinia/Gag, sera from mice infected with recombinant vaccinia virus/Gag. HIV-1-positive human serum was used as positive control.