FIG. 6.

BALB/c mice were intramuscularly injected three times with 100 μg of plasmid DNA at 0, 2, and 4 weeks. Anti-HIV-1 Gag CTL responses were measured at week 6. Splenocytes from the mice in each group were harvested and pooled, and the CTL responses specific to HIV-1 p24 peptide were measured following antigen restimulation in vitro. The target cells (p815) were pulsed with p24 peptide.