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. 2024 Jun 18;300(7):107486. doi: 10.1016/j.jbc.2024.107486

Figure 5.

Figure 5

TCDD plus Map3k1 heterozygosity tilt the balance of JNK and ERK. Eyelid tissues of WT and Map3k1+/− E15.5 embryos with or without TCDD (50 μg/kg body weight) exposure were subjected to immunohistochemistry for (A and B) p-ERK (red) and (C and D) p-JNK (red). E-cadherin (green) and Hoechst (blue) were markers of epithelial membrane and nuclei, respectively. Representative images captured with a fluorescent microscopy were shown. The (B) p-ERK and (D) p-JNK in the suprabasal epithelium of the eyelid leading edge (arrowheads), marked with dash lines, were quantified. At least three sections (N ≥ 3) per embryo and three embryos (N ≥ 3) per genotype/treatment conditions were examined. Results were shown as mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001 represents significantly different from the unexposed groups and TCDD-treated WT group (in B and D). The scale bar represent 50 μm (A and C). ERK, extracellular signal regulated kinase; JNK, Jun N-terminal kinase; MAP3K1, mitogen-activated protein kinase kinase kinase 1; TCDD, 2,3,7,8-tetrachlorodibenzo-para-dioxin.