Figure 5.

Pharmacological disruption of actin reverses FP mechanical switching in ABDTS

(A–D) Representative NIH3T3 cells expressing ABDTL with vehicle control, (E–H) ABDTL treated with latrunculin A (Lat. A), (I–L) ABDTS with vehicle control, or (M–P) ADBTS treated with latrunculin A. Images are acceptor and donor intensities, with cell outline overlaid in red and FRET efficiency and stoichiometry in cell mask.

(Q–T) ES-histograms for whole-cell populations of ABDTL with vehicle control (Q, N = 43 cells), ABDTL treated with latrunculin A (R, N = 40 cells), ABDTS with vehicle control (S, N = 39 cells), and ADBTS treated with latrunculin A (T, N = 40 cells) over 3 experimental days, where color bars indicate the cell-averaged fraction of pixels in each bin.

(U) Boxplot of fraction of pixels in each cell in the low $E$, high $S$ bin ($E<0.15,S>0.60$) for the indicated conditions. Differences between groups were detected using the Steel-Dwass test. Levels not connected by the same letter are significantly different at p < 0.05. p values are given in Note S3.