Figure 7.

Acceptor mechanical switching in VinTS is eliminated on soft substrates

(A–J) Representative vinculin^−/− MEFs expressing VinTS (A–E) or VinTS-E1015A-E1021A (F–J) on FN-coated PA gels, showing images of acceptor intensity, donor intensity, FRET efficiency in the FA mask, stoichiometry in the FA mask, and an ES-histogram of FA-masked pixels for the cell. For single-cell ES-histograms in (E) and (J), the color bars indicate pixel counts.

(K and L) ES-histograms for whole-cell populations of vinculin^−/− MEFs expressing VinTS or VinTS-E1015A-E1021A on PA gels (N = 87/92 cells over 3 experimental days for VinTS/VinTS-E1015A-E1021A), where color bars indicate the cell-averaged fraction of FA-masked pixels in each bin. The black outline for VinTS from (K) is overlaid on the histogram for VinTS-E1015A-E1021A in (L) as a guide for the eye.

(M) Boxplot of fraction of FA-masked pixels in each cell in the low $E$, high $S$ bin ($E<0.15,S>0.60$). The difference between the groups was detected using the Wilcoxon rank-sum test; n.s., not significant. p values are given in Note S3.

See also Figure S22.