TABLE 2.
MLV (Hygror) and SNV (G418r) vector titers generated by E1 cells transfected with gag-pol expression constructs
| Expt | Titera (CFU/ml)
|
|||||
|---|---|---|---|---|---|---|
| pRDI36 (SNV)
|
pLGPS (MLV)
|
pSNVgag
|
||||
| MLV vector (103) | SNV vector (103) | MLV vector (105) | SNV vector (100) | MLV vector (103) | SNV vector (101) | |
| 1 | 6.5 | 8 | 1.6 | 20 | 10 | 8.3 |
| 2 | 2.6 | 1 | 1.2 | 8 | 6.5 | 1 |
| 3 | 1.3 | 3 | 1.2 | 3 | 2.9 | 2.1 |
| 4 | 1.9 | 0.7 | 0.85 | <1 | 8.8 | 1.8 |
| 5 | 0.77 | 0.47 | 0.39 | 2 | 2.8 | 1.2 |
| Mean | 2.6 | 2.6 | 1 | 6.6 | 6.2 | 2.9 |
a
pMLVNC, pMLVgag, pSNVNC(old), pSNVNC(new), and pBSpac, MLV and SNV vector titers were <1 CFU/ml in all experiments.