Table 2. Results Obtained for the Three Samples Used for Quantificationa.
| sample code | MET-minutes from GPAQ | miR-16-5p concentration (pmol/mL) (average ±SD) |
|
|---|---|---|---|
| current assay | RT-qPCR | ||
| Sed_1 | 300 | 2.81 ± 0.06 | 3.1 ± 0.5 |
| Sed_2 | 640 | 1.18 ± 0.28 | 0.70 ± 0.15 |
| Ath_1 | N/A | 0.38 ± 0.06 | 0.30 ± 0.02 |
a
Sed_1 and Sed_2 correspond to sedentary volunteers (a higher score implies higher physical activity). Ath_1 corresponds to a professional athlete. The score for athletes training more than 150 min per day cannot be quantified by the same method. The results obtained with the proposed assay and traditional rt-PCR can be compared in the third and fourth columns.