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. 2024 Jul 18;146(30):20627–20635. doi: 10.1021/jacs.4c03092

Figure 7.

Figure 7

Synthesis and utility of ABC-caged oxytocin agonist. (a) Synthesis of PATCOT (37). (b) Pseudocolor fluorescence microscopy images of RPE-hTERT cells expressing HaloTag protein, loaded with BAPTA-JF549–HaloTag ligand AM ester,35 and incubated with 37 before (top) and after (bottom) application of uncaging light (λ = 440 nm, 1.48 mW/cm2, 1 s); scale bar: 20 μm. (c) Change in fluorescence over basal fluorescence (ΔF/F0; Z-score) vs time from the regions of interest (ROIs) denoted in panel (b) showing changes in fluorescence in response to the light pulse. (d) Plot of ΔF/F0 (Z-score) for cell incubated with DMSO (n = 72 ROIs) or 37 (n = 79 ROIs); error bars indicate mean ± SD.