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5 mM DTT does not affect electrostatic potential measurements using the Gd chelates. (A, B) Linear correlation plots of measured solvent PRE rates Γ2 for backbone 1HN nuclei of ubiquitin in the presence and absence of 5 mM DTT. (C) Correlation plot of ϕENS potentials measured for ubiquitin in the presence and absence of 5 mM DTT using the PRE rates in (A, B).
