FIG. 3.

The EMCV IRES serves as a movable targeting element for vhs-induced RNA cleavage. 5′-Cap-labeled EcoNI runoff transcripts of pSexAI IRES (A) and parental pCITE Msc/RI (B) were added to RRLs containing vhs (lanes marked vhs) and to control RRL (lanes marked RC), and samples removed at the indicated times (in minutes) were analyzed by agarose-formaldehyde gel electrophoresis as for Fig. 1. Arrows and associated numbers indicate the positions and sizes (in nucleotides) of the vhs-dependent RNA cleavage products. The numbers to the left indicate the sizes of RNA markers (lanes M) in nucleotides. (C) Diagram showing the structures of the two transcripts and the approximate locations of the vhs-induced cleavages. The shaded box represents the EMCV IRES.