Figure 5.

EHMT1 and EHMT2 as proviral factors for SARS-CoV-2 infection

(A) qPCR analysis of viral RNA.

(B) Western blot analysis of the viral N. The expression of the viral protein was evaluated by the band intensity of N, normalized to that of GAPDH. Cell lysates and culture supernatants were harvested 3 days post-infection.

(C) Western blot analysis of the inhibitory effect of UNC0642 on histone methylation. A549-hACE2 cells were treated with UNC0642 and harvested the following day. DMSO was used as a negative control.

(D) Inhibitory effect of the EHMT1/2-specific inhibitor UNC0642 on SARS-CoV-2 infection. Viral titers were measured 3 days post-inoculation at an MOI of 0.001. Treatments included pre-treatment (1 h before inoculation), co-treatment during (1 h absorption), and post-treatment (after inoculation).

(E) qPCR analysis of viral RNA in cells treated post-infection with UNC0642.

(F) Western blot analysis of viral N protein in UNC0642-treated cells. The expression of viral protein was evaluated by the band intensity of N, normalized to that of GAPDH. Data are presented as the mean ± SD of three independent experiments. Statistical analysis was performed using Dunnett’s multiple comparison test. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗∗, p < 0.0001.