FIG. 5.

Characterization of the recovered viruses by IF analysis. (A) IF analysis of repBHK and BHK cells which were infected with diluted or undiluted CFs (as indicated) collected at 4 days after transfection of ns5dSB, ns5dAB, and ns5dNB RNAs. repBHK panels show IF analysis at day 2 after infection of repBHK cells using anti-E antibodies (anti-E panels). Pairs of joined BHK panels representing the same selected field were dual labelled with anti-E (anti-E,FITC panels) and anti-NS3 antibodies (anti-NS3,TR panels). White arrows indicate selected cells positive in IF with both anti-E and anti-NS3 antibodies. (B) IF analysis using anti-E antibodies of repBHK and BHK cells infected with CFs containing complemented ns5dBsB and DENns5 viruses. Designations are as in panel A. FITC-conjugated secondary antibodies were used in all experiments with repBHK and BHK cells for IF detection of E protein, and TR-conjugated secondary antibodies were used for IF detection of NS3 protein in experiments with dual labelling in BHK cells.