FIGURE 3.

Phosphokinase signaling is starkly suppressed in aged AM. AM were collected from young and aged WT and TNF KO mice and inoculated with Spn at an MOI of 25 for 15 min. Purified protein from these samples was used in a phosphorylation array of the MAPK pathway (a) with results overlaid on the MAPK pathway (b). Any protein designated by a colored oval was tested as part of the phosphorylation array, with items in red indicating significant changes due to age and blue indicating alterations due to TNF. Statistical significance was calculated using a two‐way ANOVA with each comparison standing alone using a Fisher's LSD test. The data are presented as median with interquartile range (IQR); *p ≤ 0.05; **p ≤ 0.01. Each individual point represents the average of two technical replicates on the phosphorylation array per biological sample.