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. 2024 Mar 8;23(6):e14133. doi: 10.1111/acel.14133

FIGURE 5.

FIGURE 5

Key negative regulators are upregulated in a TNF‐dependent manner with age. Identification of Dusp1 and Ptprs in DEGs as a result of TNF status and age from the transcriptomic dataset (a). Expression levels of negative regulators Dusp1 and Ptprs from alveolar macrophages are shown in Transcripts per Million (a), protein levels of Dusp1 and Ptprs in whole lung cell lysates via western blots (b), and in isolated AM by qPCR (c). Levels of negative regulators Dusp1 and Ptprs in J774.1 cells treated with various levels of TNF via qPCR (d). Levels of negative regulators Dusp1 and Ptprs in AM from young and aged mice collected and exposed to TNF ex vivo (e). Statistical test was done using either a Kruskal–Wallis (d & e) or a Mann–Whitney test (c). The data are presented as median with interquartile range (IQR); *p ≤ 0.05; **p ≤ 0.001; ****p ≤ 0.0001. Each data point represents an individual gene (a), mouse (b & c), or well (e).