Table 1. Isolation of NDV from suspected field samples using ECEs.
| Outbreak Districts | No. of farms affected | No. of samples collected | Primary screening by rapid NDV Ag kit | Isolation of viruses using ECEs | HA test positive allantoic fluid | No of positive isolates of NDV using ECEs |
|---|---|---|---|---|---|---|
| Joypurhat | Sonali=7 | 42 | 12 | 12 | 8 | 10 (16.16%) |
| Broiler=3 | 18 | 2 | 2 | 2 | ||
| Gaibandha | Layer=6 | 36 | 8 | 8 | 5 | 8 (13.13 %) |
| Broiler=4 | 24 | 4 | 4 | 3 | ||
| Bogura | Sonali=5 | 30 | 8 | 8 | 5 | 8 (13.13 %) |
| Broiler=5 | 30 | 4 | 4 | 3 | ||
| Sirajganj | Sonali=6 | 36 | 5 | 5 | 5 | 9 (15.0%) |
| Broiler=4 | 24 | 5 | 5 | 4 | ||
| Tangail | Layer=7 | 42 | 16 | 16 | 8 | 10 (16.16%) |
| Broiler=3 | 18 | 2 | 2 | 2 | ||
| Mymensingh | Layer=4 | 24 | 4 | 4 | 3 | 7 (11.66 %) |
| Broiler=6 | 36 | 4 | 4 | 4 | ||
| Pabna | Layer=6 | 36 | 10 | 10 | 5 | 8 (13.13 %) |
| Broiler=4 | 24 | 3 | 3 | 3 | ||
| Total | 70 | 420 | 87 | 87 | 60 | 60 |
NDV-Newcastle diseases virus; ECEs-Embryonated chicken eggs; HA-Hemagglutination