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. 1999 Nov;73(11):9294–9302. doi: 10.1128/jvi.73.11.9294-9302.1999

FIG. 1.

FIG. 1

Expression of Wt-EGFR and Tr-EGFR in cellular membranes. Upper panel: Schematic representation of Wt-EGFR (extracellular domain, 647 aa; membrane anchor, 21 aa, cytoplasmic domain, 542 aa) and Tr-EGFR (extracellular domain, 647 aa; membrane anchor, 21 aa; cytoplasmic domain, 7 aa). Lower panel: Autoradiogram of a PAGE analysis of immunoprecipitates obtained with EGFR antibody from the membrane (M) and cytosolic (C) fractions of metabolically labelled 293T cells transfected with pKEx-Wt-EGFR (Wt) or pKEx-Tr-EGFR (Tr). The positions of molecular mass markers are shown on the left.