FIG. 1.

Ad vectors with incorporated AAV cassettes. The genomic structures of first-generation vectors are shown. The length of these genomes is ∼35 kb. Arrows indicate promoters. Abbreviations: Ψ, Ad packaging signal; MLV, Moloney murine leukemia virus promoter; SV40, SV40 promoter; (PA), SV40 polyadenylation signal; Tn5, bacterial promoter; p15 ori, p15a bacterial plasmid origin with the direction of leading strand-strand DNA synthesis opposite to that of neo gene transcription. The palindromic AAV ITRs (ABB′CC′A′D and D′ACC′BB′A′) are framed. The following control vectors were generated: Ad.AAV1-Δ2ITRs (containing the transgene cassette without AAV ITRs), Ad.AAV1-GC (containing the transgene cassette flanked on both sides with an 20-mer dG and dC stretch), and Ad.AAV1-Δ1ITR (containing one intact AAV ITR upstream of the transgene cassette and an AAV ITR deleted for most of A′, the complete BB′/CC′ regions, and most of A located downstream of the transgene cassette).