Table 3. Variation during helical twisting measured by HHPs, the number of protomer pairs per HHP, and the MADf observed for protomers representing F-actin structures within long bare actin filaments, as probed by HS-AFM.
The total number of HHPs observed was 2226. The total number of protomer pairs used for estimating MADf was calculated by multiplying the number of protomer pairs per HHP by the number of HHPs.
| The number of protomer pairs per HHP | HHPs (nm) | MADf (nm) | The number of HHPs (proportion) | The total number of protomer pairs |
|---|---|---|---|---|
| 4.5 | 28.6±1.4 | 6.3±0.3 | 5 (0.2%) | 22.5 |
| 5.5 | 29.3±1.8 | 5.3±0.3 | 182 (8.2%) | 1001 |
| 6.5 | 33.1±1.3 | 5.1±0.2 | 1355 (60.9%) | 8807.5 |
| 7.5 | 37.2±2.0 | 5.0±0.3 | 624 (28.0%) | 4680 |
| 8.5 | 39.1±2.4 | 4.6±0.3 | 60 (2.7%) | 510 |