Table 4. Quantifying time constants (t1, t2) obtained from fitting individual ACF curves.
The t1 and t2 values are associated with first and second time constants of axial distance (AD) between two adjacent actin protomers within F-ADP-actin and F-ADP.Pi-actin segments. These time constants reflect the time decay in the autocorrelation of AD, recorded experimentally at high temporal resolution using HS-AFM. Related to Figure 3—figure supplement 1.
| Experimental conditions | t1 (s) | t2 (s) |
|---|---|---|
|
F-ADP-actin (200ms/frame) |
0.4±0.2 (N=82) |
0.5±0.7 (N=80) |
|
F-ADP-actin (100ms/frame) |
0.3±0.3 (N=78) |
1.2±1.8 (N=78) |
|
F-ADP.Pi-actin (100ms/frame) |
0.2±0.3 (N=152) |
0.5±1.1 (N=141) |