Table 7. A summary of peak heights and HHPs obtained from the histograms in Figure 8.
Single and double Gaussian fitting methods were applied to the data. The significant difference between the peaks in the Gaussian fitting was statistically evaluated and indicated using an F-test. ‘ND’ indicates cases where the information was not determined.
| Peak Height (nm) | Half Helical Pitch (nm) | Frequency | |||
|---|---|---|---|---|---|
| Peak 1(proportion)[F-test, p] | Peak 2(proportion)[F-test, p] | Peak 1(proportion)[F-test, p] | Peak 2(proportion)[F-test, p] | ||
| (A) 2000 nM cofilin, 0.5 mM ATP/ on DPPC/DPTAP (75/25 wt%) | 8.2±0.8 (100%) |
ND | 34.3±2.6 (100%) |
ND | Height: 1930 HHP: 1556 |
| (B) 300 nM cofilin, 0.5 mM ATP/ on DPPC/DPTAP (90/10 wt%) | 9.2±0.4 (47.0%) [0.0204] |
10.5±0.7 (53.0%) [0.0204] |
28.5±3.0 (55.1%) [0.0135] |
37.4±3.6 (44.9%) [0.0135] |
Height: 2212 HHP: 1780 |
| (C) 300 nM cofilin, 1 mM ADP/ on DPPC/DPTAP (90/10 wt%) | 8.4±0.5 (54.8%) [0.0081] |
9.9±0.7 (45.2%) [0.0081] |
30.1±3.8 (70.3%) [0.0195] |
37.8±2.9 (29.7%) [0.0195] |
Height: 3183 HHP: 2779 |
| (D) 75 nM cofilin, 1 mM ATP, 10 mM Pi/ on DPPC/DPTAP (90/10 wt%) | 8.3±0.7 (55.3%) [1.48x10–6] |
11.0±1.0 (44.7%) [1.48x10–6] |
30.3±2.9 (25.2%) [0.0002] |
38.7±3.6 (74.8%) [0.0002] |
Height: 2316 HHP: 1865 |