FIGURE 2.

Starvation impairs the migration and proliferation capacity of ABCC2 high‐expression cell lines. (A) The knockout efficiency of ABCC2 was determined by western blot and immunofluorescence in BGC‐823 and SGC7‐901 cells. Green: ABCC2 immunofluorescence; blue: DAPI. (B–E) Proliferation and migration assays were performed to examine the biological function in ABCC2‐ knockout BGC‐823 and SGC‐7901 cells. (F) The efficiency of ABCC2 overexpression was determined by western blot and immunofluorescence in MGC‐803 and AGS cells. Green: ABCC2 immunofluorescence; blue: DAPI. (G, H) Proliferation and migration assays were performed to examine the biological function in ABCC2 overexpressed MGC803 and AGS cells. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the vehicle control (NC group); Student's t‐test.