Fig. 1. LDAH overexpression protects against atherosclerosis.

A LDAH transgene design with the colony stimulating factor 1 receptor (Csf1r) promoter and intron 2 that contains the enhancer element FIRE. B LDAH RNA expression in LDAH-Tg (Tg, blue bars) and LDAH-WT (WT, gray bars) peritoneal macrophages (PM) that remained untreated or were treated with acetylated LDL (acLDL, 50 μg/ml). Data are presented as mean ± SEM of 3 independent samples. ****P < 0.0001, two-way ANOVA followed by Tukey’s test. C Western blot showing increased LDAH protein in transgenic peritoneal macrophages (PM) and bone marrow-derived macrophages (BMM). The panel shows n = 3 biological replicates representative of at least 3 independent experiments. D, E Representative H&E images of aortic root sections and quantification of atherosclerotic lesion size in 20-week-old male (D) and female (E) Ldah^0/0ApoE^−/− (WT, n = 10 male and 11 female, gray dots) and Ldah^Tg/0ApoE^−/− (Tg, n = 10 male and 17 female, blue dots) littermates fed standard chow. Data are presented as mean ± SEM. All bars= 200 μm. *P = 0.0433 by two-tailed Mann-Whitney U test (panel D); **P = 0.0013 by two-tailed unpaired t-test (E). Source data are provided as a Source Data file.