Figure 2.

Morphological analyses that have been used for organoids

(A) Morphological analyses of organoids include organoid volume, organoid neural rosette number, diameter, perimeter, lumen area, ventricular zone area, ventricular zone + lumen area, length of basal membrane, and length of apical membrane.

(B) Cellular architectural analyses of organoids include the measurements of thickness of cellular layers resembling cortical layers in the brain as identified by common developmental markers. The thicknesses of cell layers were obtained by three measurements equally distanced by 45°.