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Lack of RNA replication of PV chimeras containing CVB3 3Dpol. (A) Schematic representation of a chimeric PV replicon in which the nonstructural genes have been replaced with a luciferase reporter gene. (B) Time course of RNA replication measured by luciferase accumulation following RNA transfection of HeLa cells at 37°C. (C) Time course of RNA replication measured by luciferase accumulation following RNA transfection of HeLa cells at 33°C.
