FIG. 5.

RNA replication of a PV-replicon chimera containing CVB3 3CD. (A) A schematic representation of a full-length chimeric PV1 genome structure and viral protein organization similar to that in Fig. 1A is shown. The 3CD coding sequences and 3′ NCR of PV1 genome were replaced with those of CVB3. (B) A schematic representation of a chimeric PV replicon containing CVB3 3CD and 3′ NCR is shown. (C) Time course of RNA replication measured by luciferase accumulation following RNA transfection of HeLa cells at 37°C. (D) Time course of RNA replication measured by luciferase accumulation following RNA transfection of HeLa cells at 33°C.