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In vitro translation and processing of PV1, CVB3, and chimeric T7 transcripts. Full-length transcripts from wild-type PV1 cDNA (lanes 3 to 6), chimeric PV1-CVB3 3CD cDNA (lanes 7 to 10), and wild-type CVB3 cDNA (lanes 11 to 14) were used to program translation in HeLa S10 extracts (supplemented with a ribosomal salt wash from HeLa cells) in the presence of [35S]methionine. Reactions were analyzed as described in the legend to Fig. 2.
