FIG. 7.

Inability of CVB3 5′CL to rescue temperature sensitivity of PV1-CVB3 3CD replicon. (A) Schematic representation of a chimeric PV replicon in which the structural genes have been replaced with a luciferase reporter gene is presented. The first ∼100 5′ nt, 3CD coding region, and 3′ NCR of PV1 sequences were replaced with CVB3 sequences. (B) Time course of RNA replication measured by luciferase accumulation following RNA transfection of HeLa cells at 33°C. In this experiment, pRLuc31-2Cpro, which has a lethal mutation in 2C and does not replicate (25), was used as a negative RNA replication control.