TABLE 1.

Plasmids generated by PCR site-directed mutagenesis^a

Plasmid [virus]	Parent plasmid	Nucleotide change	Position	Amino acid change	Infectious status
pEXC-3D(AgeI) [PV]	pEXC-3Db	ACC	5985–5987	Q→T	NAc
pCVB3(NdeI)	pCVB3	CATATG	5356–5361	V→H; Q→M	NDd
pT5T-3CD (AgeI) [CV]	pT5T-3CD	ACC	5908–5910	Q→T	NA
pEXC-PC-3CD [PV-CV]	pEXC-3CD(AgeI)	CAA	5908–5910	T→Q	NA
pT7PV1(Bsu36I)	pT7-PV1	CTGA	5417–5420	I→L	Yes
pLucPV(SnaBI)	pLucPV	T,T	109,113	NA	NAe
pCVB3(SnaBI)	pCVB3	T,C	112,114	NA	Yes

^aReference 20. 

^bFrom reference 46. 

^cNA, not applicable. 

^dND, not determined. 

^eReplicon exhibited wild-type RNA replication kinetics at 37 and 33°C as measured by luciferase assay.