. 1999 Nov;73(11):9413–9421. doi: 10.1128/jvi.73.11.9413-9421.1999

TABLE 2.

Plasmids generated by two-fragment ligation reactions

Plasmid [virus]	Vector plasmid	Insert plasmid	Restriction enzyme(s)
pT5T-3CD [CV]	pT5T-3D^a	pCVB3 (NdeI)	NdeI, SalI
pEXC-3CD(AgeI) [PV]	pEXC-3D(AgeI)	pT5T-3CD(AgeI)	SpeI^bAgeI (vector); SalI^bAgeI (insert)
pET15-(PC)3CD(AgeI) [PV-CV]	pET15 3CDμ10^c	pEXC-3CD(AgeI)	DraIII, XhoI (vector) DraIII, SalI (insert)
pT7PV1-CV_3CD	pT7PV1(Bsu36I)	pCVB3	Bsu36I, SalI
pT7PV1-CV_3D	pPT7-PV1	pEXC-PC-3CD	BglII, SalI
pLucPV-CV_3D	pLucPV	pEXC-PC-3CD	BglII, SalI
pLucPV-CV_3CD	pLucPV	pT7PV1-CV_3CD	SpeI, SalI
pCV_CLLucPV	pCVB3(SnaBI)	pLucPV(SnaBI)	SnaBI, SalI
pCV_CLLucPV-CV_3CD	pCV_CLLucPV	pLucPV-CV_3CD	SpeI, SalI
pLucPVΔ3D^d	pLucPV	NA	HindIII

From reference 18.

Plasmids were first digested with the indicated enzyme; overhanging ends were filled in with Klenow enzyme and then subsequently digested with AgeI.

From reference 4.

Plasmid resulted from a one-fragment ligation in which the internal HindIII fragment within 3D was deleted.