FIG. 2.

Immunoblot analysis of the various Rep78 proteins expressed from the HIV LTR in transiently transfected 293 cells. Mutant and wt pHIV-78 constructs were transfected into 293 cells at 32, 37, and 39°C as described in Materials and Methods. A representative blot of protein expression is shown. With the exception of D40,42,44A, all lysates were prepared from cells transfected at 37°C. Blots were hybridized with a monoclonal antibody that recognizes all four Rep proteins. All lanes were loaded with 15 μl of cellular extract. ∗, proteins were detected after long-term exposure of blots.