FIG. 6.

Binding, trs endonuclease, and helicase activities of D412A-68 and Rep68H6 as a function of protein concentration. (A) Standard binding reactions were performed as described for Fig. 5A and carried out in triplicate with 1 nM substrate (³²P-TR) and the indicated concentration of each protein. (B) Standard nicking reactions were performed as described for Fig. 5B and carried out in triplicate with 1 nM ³²P-TR substrate and the indicated concentration of each protein. (C) Standard helicase reactions were performed as described for Fig. 5C and carried out four times in nicking buffer containing 1 nM substrate (M13/24) and the indicated concentration of protein.