Fig. 7. The RAG sequence is important for aminoacylation state recognition.

a Left: Schematic diagram of the Mtb-ileS T-box with the NCAA/T-box and RAG sequence regions shown inside the black box. Right, schematic diagrams showing the RAG sequence region (GAG) (top) and the mutations introduced in the RAG Sequence mutant (bottom). The interactions affected are circled in green in the mutant diagram. b FRET efficiency histogram of the data with the Linker Mutant. Fitted populations using consensus HMM modeling are plotted as in Fig. 2c. c Transition density plot constructed using the idealized Viterbi paths, as described in Fig. 2d. d–f Dwell time survival plots and fitting result using a single or double exponential decay function for the (d) 0.4 FRET state, (e) 0.7 FRET state and the (f) 0.9 FRET state. Data and fitting results are presented as described in Fig. 2e. N in each plot reports the number of traces or events included in the analysis. Source data are provided as a Source Data file.