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Branched PACE and polyplexes. (A) Schematic illustration of PACE and branched PACE (bPACE), the consisting monomers, and formation of mRNA/bPACE polyplexes. (B) Degree of branching (DB) of bPACEs. (C) Monomer contents of bPACEs and PACEs. (D) Hydrodynamic size (z-average) and polydispersity index (PDI) E) Zeta-potential of polyplexes formulated with bPACE.
