Fig. 5.

FLuc mRNA was delivered using bPACE polyplexes by IT or IV administration, and Summarized effects of PACE branching. Representative IVIS images of luminescence in organs after (A) IT and (B) IV delivery. Luciferase expression in tissue quantified by luminescence assay for (C) IT delivery (lungs) and (D) IV delivery (lung, spleen, liver, kidney). (Polyplexes were formulated at the ratio of 1:25 (mRNA:polymer; w/w), n = 4, mean ± S.D., Statistical significance was accessed with One-Way ANOVA test, *p < 0.05, **p<0.01). (E) Heat map and diagram visualizing the effects of degree of branching. (^aTitration: calculated buffer capacity obtained from acid-base titration (Fig. 2B, pH 5 to pH 6); ^bTNS assay: buffer capacity calculated from TNS assay intensity change (Fig. 2C, pH 6.5 to pH 7.5) ^cAssociation: mRNA-bPACE association strength evaluated from the dissociation half-life obtained from fig. 2D; ^dShelf-life: the day with 0% GFP+ in the extrapolated plot from Fig. 4B; ^e in vitro mRNA transfection from Fig.3D; ^fin vivo mRNA transfection from Fig. 5C. (The data in heat map were normalized to show clear trends, detail information on data collection and values were listed on Table S6–8.)