TABLE 1.

PFAS evaluated in present study during initial transcriptomic investigation. PFAS numbering was established after completion of experiments to correspond to increasing number of differentially expressed genes up to PFAS 13. BMD₈₀ was determined during developmental toxicity testing (A. in Figure 1) by Truong et al. (2022) for 14 PFAS, and exposure concentration for the initial transcriptomic investigation in this study (Methods Section 2.4) was determined through a series experiments to confirm the target effective concentration.

PFAS number	PFAS name	Common abbreviation	Stock concentration in DMSO (mM)	CAS	BMD80 (µM)	Exposure concentration (µM) (methods 2.4)	Chemical structure
1	9-Chloro-perfluorononanoic acid	—	30	865-79-2	81.4	100
2	7H-Perfluoro-4-methyl-3,6-dioxaoctanesulfonic acid	Nafion Byproduct 2	22	7,49,836-20-2	28.2 a	30.0
3	Potassium Perfluorooctane sulfonate	PFOS	30	2,795-39-3	13.8	13.8
4	Perfluoro-3,6,9-trioxatridecanoic acid	—	30	3,30,562-41-9	92.2	50.0
5	Perfluoroheptane sulfonic acid	PFHpS	20	375-92-8	98.7	100
6	N-Ethyl perfluorooctane sulfonamide	EtFOSA	30	4,151-50-2	>100	100
7	1-(Perfluorofluorooctyl) propane-2,3-diol	—	5	94,159-84-9	39.7	39.7
8	1H,1H,11H,11H-Perfluorotetraethylene glycol	—	30	3,30,562-44-2	28.6	53.0
9	2,2,3,3-Tetrafluoropropyl acrylate	—	30	7,383-71-3	56.0	62.0
10	Perfluorooctane sulfonamide	FOSA	30	754-91-6	3.6	8.0
11	Perfluorohexane sulfonamide	FHxSA	30	41,997-13-1	12.5	28.0
12	3H-Perfluoro-2,2,4,4-tetrahydroxypentane	—	30	77,953-71-0	41.8	100
13	1H,1H,10H,10H-Perfluorodecane-1,10-diol	—	30	754–96-1	14.0	25.0
14	1H,1H,5H,5H-Perfluoro-1,5-pentanediol diacrylate	—	30	678-95-5	12.5	20.0
15	1H,1H,6H,6H-Perfluorohexane-1,6-diol diacrylate	—	30	2,264-01-9	15.3	20.0

^a PFAS evaluated as methanol stock during initial developmental toxicity testing.