Fig. 5. Activation of ARF6 and expression of immunosuppressive genes downstream of IFNγ.

a Representative images of immunohistochemical (IHC) detection of PD-L1 expression (brown) in ARF6^WT tumours and summary of PD-L1 detection in n = 10 tumours of each genotype tested, two-sided Fisher’s exact test. Thymus and lymph node are used as controls, (b–h) IFNγ-induced expression. b Flow cytometric detection of tumour cell surface and total protein, ARF6^WT n = 6, ARF6^f/f n = 7 biologically independent tumour cell lines of each genotype. c Western blot for indicated proteins, n = 3 biologically independent tumour cell lines of each genotype. d Quantitative RT-PCR analysis for Cd274 mRNA, three biologically independent tumour cell lines of each genotype, n = 3 replicates per cell line, per treatment condition. e Western blot for indicated proteins in UACC.62 cells, n = 3 biologically independent experiments. f Western blot for indicated proteins in early-passage murine tumour cells, n = 3 biologically independent tumour cell lines of each genotype. g Quantitative RT-PCR for Ido1 mRNA, n = 3 biologically independent tumour cell lines of each genotype, n = 3 replicates per cell line per condition. experiments. h Western blot for indicated proteins in UACC.62, n = 3 biologically independent experiments. b, c, d, g Two-way ANOVA with Tukey’s multiple comparisons test. e, h Two-tailed, ratio paired t-test. b–d, e, g, h Solid line within data points = mean. See also Supplementary Fig. 5. Source data are provided as a Source Data file.